2019-02-19 20:22:162025-05-13 08:07:12
locus
BSU09420
BSU_09420
outlinks
bsu
BSU09420
BSU_09420
Gene
Phenotypes of a mutant
a ''[[gene|cwlO]] [[gene|lytE]]'' mutant is not viable [Pubmed|17581128,22139507]
growth defect at high temperature [Pubmed|21541672]
inactivation of ''[[gene|lytE]]'' strongly restores beta-lactam resistance in a ''[[gene|sigM]]'' mutant by delaying cell lysis [Pubmed|22211522]
a ''[[gene|lytE]]'' mutation is synthetically lethal with ''[[gene|ftsE]]'' and ''[[gene|ftsX]]'' mutation (due to a lack of autolysin activity) [Pubmed|23869552,23855774]
a ''[[gene|lytE]]'' mutation increases the cell separation defect of a ''[[gene|lytF]]'' mutant [Pubmed|23855774]
cells are thinner (reduced diameter) as compared to the wild type [Pubmed|23869552]
reduced colony size with accumulation of dead cells in the colonies [pubmed|29114240]
a ''[[gene|cwlO]] [[gene|lytE]]'' mutant is not viable [Pubmed|17581128,22139507]
a [[gene|ugtP]] [[gene|lytE]] double mutant has a severe [SW|cell shape] defect, thi can be suppressed by mutations resulting in reduced expression or activity of [[protein|PonA|PbpA]] [pubmed|33087775]
growth defect at high temperature [Pubmed|21541672]
inactivation of ''[[gene|lytE]]'' strongly restores beta-lactam resistance in a ''[[gene|sigM]]'' mutant by delaying cell lysis [Pubmed|22211522]
synthetically lethal with ''[[gene|ftsE]]'' and ''[[gene|ftsX]]'' mutation (due to a lack of autolysin activity) [Pubmed|23869552,23855774]
synthetically lethal with [[gene|sweC]] and [[gene|sweD]], this can be suppressed by point mutations in [[gene|ftsE]] or [[gene|ftsX]] [pubmed|31437162]
a ''[[gene|lytE]]'' mutation increases the cell separation defect of a ''[[gene|lytF]]'' mutant [Pubmed|23855774]
cells are thinner (reduced diameter) as compared to the wild type [Pubmed|23869552]
reduced colony size with accumulation of dead cells in the colonies [pubmed|29114240]
The protein
[SW|Domains]
contains three N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]
C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507]
contains three N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]
C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507]
3 [SW|LysM domain]s (aa 26-69, aa 86-129, aa 149-192) (according to UniProt)
Biological materials
Mutant
1A790 ( ''lytE''::''cat''), [Pubmed|9457885], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A790&Search=1A790 BGSC]
1A792 ( ''lytE''::''cat''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
1A1024 ( ''lytE''::''spec''), [Pubmed|20400548], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1024&Search=1A1024 BGSC]
BKE09420 ([[gene|lytE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09420 BGSC] and in [SW|Jrg Stlke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG
BKK09420 ([[gene|lytE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG
1A790 ( ''lytE''::''cat''), [Pubmed|9457885], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A790&Search=1A790 BGSC]
1A792 ( ''lytE''::''cat''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
1A1024 ( ''lytE''::''spec''), [Pubmed|20400548], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1024&Search=1A1024 BGSC]
BKE09420 ([[gene|lytE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09420 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG
BKK09420 ([[gene|lytE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCTCCCCAAATG, downstream forward: _UP4_TAATTTTTAGAGAAAACCCG
References
Original publications